I. Basic Product Information

Product Name: High-Stability Fluorescent Microspheres
Product Aliases: Nanofluorescent Beads, Fluorescent Nanospheres
Product Grade: Research Grade

Nanofluorescent beads (fluorescent microspheres) are spherical particles that uniformly encapsulate fluorescent dyes (or quantum dots, etc.) in polymer (such as polystyrene) or silica matrices. They range in size from the nanometer scale (typically 10nm to several micrometers) and possess stable fluorescent properties and excellent dispersibility.

This product is specifically designed for high-end biomedical research, optical instrument calibration, and nanotechnology applications. Its core advantages include high particle size uniformity, strong fluorescence intensity, and excellent photostability, which can effectively improve the reliability of experimental data. It is an ideal choice for fluorescent labeling and detection in scientific research.

II. Technical Specification Standards

2.1 Basic Parameter Range

Parameter Category	Specific Specifications	Description
Particle Size Range	20nm-500μm	Integrates two expressions from the original document (40nm-500nm and 20nm-500μm) to cover a wider range of size requirements
Surface Modification	Carboxyl Modification, Amino Modification	The two modification types can meet the conjugation needs of different biomolecules (such as antibodies, antigens, nucleic acids)
Storage Conditions	Recommended storage at 2-8℃ in the dark	Supplemented based on the characteristics of similar products, which can effectively extend the product shelf life
Shelf Life	12 months in unopened condition	Supplemented in accordance with general standards for research-grade products

2.2 Detailed Parameters of Color Models

Fluorescence Color	Particle Size Range	Maximum Excitation Wavelength	Maximum Emission Wavelength	Application Scenarios
Red	20nm-500μm	632nm	680nm	Suitable for experiments requiring avoidance of biological autofluorescence interference, such as deep tissue imaging
Green	20nm-500μm	488nm	520nm	Preferred for routine fluorescence imaging, compatible with the 488nm laser channel of most fluorescence microscopes
Blue	20nm-500μm	405nm	425nm	Suitable for the blue channel in multicolor labeling experiments, with no overlap with other fluorescent colors
Orange	20nm-500μm	380nm	610nm	Suitable for ultraviolet excitation scenarios, with strong penetration of fluorescent signals

III. Product Advantage Analysis

3.1 Performance Advantages

Excellent Optical Performance: High fluorescence intensity and strong signal strength enable accurate detection at low concentrations; excellent photostability and outstanding anti-photobleaching ability support long-term continuous observation.
Stable Physical Properties: Good particle size uniformity (CV value usually < 5%), excellent dispersibility, and low tendency to aggregate; high sphericity and regular morphology ensure the consistency of experimental results.
Controllable Surface Properties: Offers two modification options (carboxyl and amino), with stable surface active group density, facilitating subsequent biomolecule conjugation with high coupling efficiency.

3.2 Application Advantages

High Flexibility: Multiple color and particle size combinations are available to flexibly match specific experimental requirements.
Wide Compatibility: Compatible with various experimental platforms such as flow cytometry, fluorescence microscopy, and immunoassays, without the need for special equipment modification.
Reliable Quality: Adheres to strict quality control standards for research-grade products, with good batch-to-batch stability and high experimental reproducibility.

IV. Recommended Application Fields

Application Field	Specific Application Scenarios	Recommended Models
Biomedical Research	Cell Tracking, Cell Migration Observation, Cell Differentiation Research	Green (488nm excitation), Red (632nm excitation)
Immunoassay and Diagnosis	ELISA Detection, Immunochromatographic Test Strips, Western Blotting	Blue (405nm excitation), Green (488nm excitation)
Flow Cytometry	Cell Counting, Cell Sorting, Detection of Cell Surface Markers	Green (488nm excitation), Orange (380nm excitation)
Optical Instrument Calibration	Fluorescence Microscope Calibration, Flow Cytometer Sensitivity Calibration	Red (632nm excitation), Green (488nm excitation)
Nanotechnology Research	Nanoparticle Dispersibility Testing, Nanocarrier Performance Evaluation	Multicolor combination

If you’re interested, have any questions, or have specific customization requirements, please feel free to contact us at inquiry@atomfair.com.

Photonics & Optoelectronic Materials