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40 L CO2 Incubator, Professional Cell Culture Series with IR CO2 Control
Research-grade laboratory product
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LABORATORY PROCUREMENT SUPPORT
For model selection, accessory matching, installation-space review, power-supply confirmation or configuration support, contact our technical sales team.
E-MAIL: inquiry@atomfair.com
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Supplier: Atomfair
Brand: ATOMFAIR®
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The incubator requires a stable AC 220 V, 50 Hz power supply and an ambient temperature range of +5 to +30 °C for proper operation. The unit must be positioned with adequate clearance for the 590 × 440 × 576 mm external dimensions and kept upright during transport.
- Power Supply Requirement: Confirm the available power supply matches the specified AC 220 V, 50 Hz requirement before installation.
- Ambient Temperature Limit: Operate the incubator only within the stated working ambient temperature range of +5 to +30 °C to maintain performance.
- Sterilization Cycle Constraint: The 90 °C high-temperature and high-humidity sterilization cycle must be used to treat the chamber and internal components.
- Transport Orientation: Keep the packaged unit upright and use suitable lifting equipment to reduce movement and impact risk during transport.
This procedure covers the initial setup, sterilization, and routine operation of the incubator for cell culture workflows. Follow these steps to ensure safe and repeatable incubation conditions.
Required Equipment: AC 220 V, 50 Hz power supply, CO2 gas supply with regulator, Cleaning supplies for sterilization cycle
- Verify Installation Space
Confirm the installation area accommodates the 590 × 440 × 576 mm external dimensions and provides access to the power supply. - Connect Power and Gas
Connect the incubator to the specified AC 220 V, 50 Hz power source and attach the CO2 gas supply with a regulator. - Run Sterilization Cycle
Initiate the 90 °C high-temperature and high-humidity sterilization cycle to treat the chamber and internal components before use. - Set Temperature and CO2
Set the desired temperature (RT + 3 to 55 °C) and CO2 concentration (0 to 20%) using the LCD display and controller. - Load Samples
Place the cell culture samples on the shelves, ensuring the chamber, sensors, and filters remain accessible for monitoring. - Monitor Alarms
Monitor the over-temperature, CO2 deviation, and prolonged door opening alarms to identify abnormal conditions during operation.
What is the CO2 recovery time after a door opening and how does the infrared sensor maintain accuracy?
The CO2 recovery time is no more than 3 minutes to return to 5% CO2 after a 30-second door opening. The infrared CO2 sensor provides ±0.1% control accuracy, enabling stable CO2 control and faster recovery in frequent door-opening workflows.
What are the power supply and ambient temperature requirements for installing this 40 L CO2 incubator?
The incubator requires AC 220 V, 50 Hz power supply and an ambient working temperature range of +5 to +30 °C. The external dimensions are 590 × 440 × 576 mm, so space must be confirmed before installation. The temperature control range is RT+3 to 55 °C, with ±0.3 °C uniformity at 37 °C.
How does the 90 °C sterilization cycle work and what optional accessories are available for contamination control?
The incubator features a 90 °C high-temperature and high-humidity sterilization cycle that treats the chamber and internal components. Additionally, microbial filtration at the CO2 inlet and optional HEPA filtration support cleaner chamber conditions. Door heating reduces condensation on the inner glass door, supporting contamination-sensitive cell culture workflows.
The 40 L CO2 Incubator (AF-CO2C-040) provides infrared CO2 sensing with ±0.1% accuracy and rapid recovery (≤3 min to 5% CO2 after 30 s door opening), combined with 90 °C high-temperature sterilization for contamination control. Its gas-jacket heating delivers ±0.3 °C uniformity at 37 °C, but operation is constrained by a 220 V / 50 Hz power supply requirement and a narrow ambient temperature range of +5 to +30 °C.
Positive
- Infrared CO2 sensing with fast recovery: Infrared sensor achieves ±0.1% CO2 control accuracy and recovers to 5% within 3 minutes after a 30-second door opening, supporting stable conditions in frequent-access workflows.
- 90 °C high-temperature sterilization cycle: The chamber and internal components can be sterilized at 90 °C with high humidity, providing a repeatable decontamination method for contamination-sensitive cell culture.
Trade-offs
- Fixed AC 220 V / 50 Hz power requirement: The incubator requires a 220 V, 50 Hz supply; laboratories with different mains voltages or frequencies will need a step-up transformer or frequency converter, adding infrastructure cost.
- Narrow ambient temperature operating range: The unit is specified for ambient temperatures of +5 to +30 °C; operation outside this range may degrade temperature uniformity or require additional environmental control.
Every advanced material, component, equipment, and instrument in our catalog is backed by rigorous testing. We maintain strict internal quality management frameworks and align with CE conformity metrics to deliver transparent, reproducible performance data via our public open-science repository.
To request raw batch performance data, submit formal vendor registration paperwork, or execute a fast-turnaround R&D manufacturing loop, contact us at inquiry@atomfair.com.
Item is dispatched under the Atomfair Shipping & Delivery Framework (Free worldwide shipping on orders over $59 USD excl. heavy equipment). Return is governed by the Atomfair Return & Refund Policy (7-day technical return window).







