PEG-NH 2-modified upconverting nanoparticles(greenyellow light), 10 mgProduct Type: Upconverting nanoparticle
Research-grade laboratory product
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LABORATORY PROCUREMENT SUPPORT
For model selection, material format, solvent, concentration or configuration confirmation, contact our technical sales team.
E-MAIL: inquiry@atomfair.com
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Manufacturer: Atomfair LLC
Brand: ATOMFAIR®
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Manufacturer: Atomfair LLC
Brand: ATOMFAIR®
How does the 35 nm particle size of these PEG-NH2-modified upconverting nanoparticles affect their suitability for cellular imaging compared to larger nanoparticles?
The 35 nm particle size is small enough to facilitate cellular uptake and endocytosis, while the PEG-NH2 modification enhances colloidal stability in water. The source specifies a NaYREF4 host with dual emission peaks at 545/660 nm, but does not provide comparative data on brightness or uptake efficiency relative to other sizes. This size is typical for bioimaging applications where small dimensions are advantageous for intracellular delivery.
What surface chemistry do these upconverting nanoparticles have, and how can they be conjugated to antibodies or other biomolecules?
The nanoparticles are PEG-NH2-modified, providing primary amine groups on the surface. These amines enable covalent conjugation to biomolecules via standard crosslinkers such as NHS esters or glutaraldehyde. The source confirms the PEG-NH2 modification and water solubility, making them compatible with common bioconjugation protocols for targeted imaging or sensing.
What storage conditions are recommended to maintain the colloidal stability and optical properties of these upconverting nanoparticles?
The source advises matching the product format (powder or dispersion) to the intended storage practice. The nanoparticles are supplied in water with PEG-NH2 modification, which enhances stability. No specific temperature or light sensitivity is stated; for detailed recommendations, users should contact the manufacturer's technical sales team. Standard practice for upconverting nanoparticles includes storage in a cool, dark place.
The PEG-NH2-modified upconverting nanoparticles (NaYREF4, 35 nm, dual emission at 545/660 nm) in a 10 mg package provide a water-dispersible, biofunctional luminescent probe for NIR-to-visible upconversion imaging, but require careful handling to maintain surface chemistry and excitation efficiency.
Positive
- Dual emission for ratiometric sensing: Two distinct emission peaks (545/660 nm) under single NIR excitation enable internal referencing in biological or materials imaging, reducing artifacts from concentration or excitation fluctuations.
- Water-dispersible PEG-NH2 surface: PEG-amine functionalization provides colloidal stability in aqueous buffers and a reactive anchor for bioconjugation, facilitating integration into immunoassays or cellular labeling workflows without organic solvents.
Trade-offs
- Moderate upconversion efficiency: Upconversion quantum yield is inherently low compared to downshifting fluorophores; excitation power density and NIR laser source coherence must be carefully controlled to achieve usable signal in low-light detection systems.
- Surface stability depends on storage: PEG-NH2 layers may hydrolyze or degrade under prolonged room-temperature storage or in acidic conditions; the product should be stored in neutral aqueous solution at 4 °C to preserve colloidal and functional integrity.
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To request raw batch performance data, submit formal vendor registration paperwork, or execute a fast-turnaround R&D manufacturing loop, contact us at inquiry@atomfair.com.
Item is dispatched under the Atomfair Shipping & Delivery Framework (Free worldwide shipping on orders over $59 USD excl. heavy equipment). Return is governed by the Atomfair Return & Refund Policy (7-day technical return window).
