Description
TopPette Single-channel 0.1-2.5 µL PipetteProduct Type: Manual pipette
Research-grade liquid handling platform
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TAILORED SOLUTIONS FOR RESEARCH
For bulk orders, OEM requirements, accessory matching, platform selection or configuration confirmation, contact our engineering sales team.
EMAIL: inquiry@atomfair.com
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Supplier: Atomfair
Brand: ATOMFAIR®
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The pipette requires periodic calibration to maintain systematic and random error within specified tolerances. The lower half is autoclavable for sterilization, but compatible tips must be selected to ensure proper fit and seal.
- Calibration Requirement: Calibrate the pipette per ISO 8655-2 using the test volumes 2.5, 1.25, and 0.25 µL to verify systematic and random error remain within the listed limits.
- Sterilization Constraint: Only the lower half of the pipette is autoclavable; ensure the upper assembly is not subjected to steam sterilization.
- Tip Compatibility: Use only tips validated for 0.1–2.5 µL volume range to guarantee accurate aspiration and dispensing.
How does the accuracy of the TopPette 0.1-2.5 µL pipette vary across its volume range, particularly at the lower end near 0.25 µL?
The systematic error increases significantly at lower volumes. At 2.5 µL, systematic error is ±0.04 µL (±1.4%); at 1.25 µL, ±0.03 µL (±2.5%); at 0.25 µL, ±0.03 µL (±12.0%). Random error is ±0.02 µL across all test volumes, but relative random error increases from 0.7% at 2.5 µL to 6.0% at 0.25 µL. This indicates that routine accuracy at the lowest end of the range requires careful calibration and method validation.
Is the entire TopPette pipette autoclavable, or only specific parts, and what are the implications for sterilization workflow?
Only the lower half of the pipette is autoclavable where indicated. Full sterilization of the entire assembly is not specified. Users must confirm compatible tips and sterilization requirements before ordering. For routine decontamination, the upper assembly should be cleaned by other methods such as ethanol wiping, which affects laboratory protocols for nuclease-free conditions in PCR or qPCR setups.
What calibration procedure or reference standard should be used for the TopPette single-channel pipette, and how does the user calibration affect routine accuracy verification?
The pipette is calibrated according to ISO 8655-2 user calibration reference as indicated in the technical table. The manufacturer recommends reviewing systematic and random error at the specific test volumes (2.5, 1.25, 0.25 µL) for routine accuracy verification. Users should perform user calibration per ISO 8655-2, especially when operating at the lower end of the volume range where relative error is highest. No specific frequency is stated, but periodic verification is implied.
The TopPette Single-channel 0.1-2.5 µL pipette offers 0.002 µL increment for precise sub-microliter dispensing and lower-half autoclavability for decontamination; however, accuracy degrades significantly at 0.25 µL (±12% systematic error) and full sterilization is not supported.
Positive
- Fine increment for precise volume selection: The 0.002 µL increment allows fine adjustment in the sub-microliter range, enabling accurate pipetting for applications such as PCR setup and serial dilution.
- Partial autoclavability for decontamination: The lower half of the pipette is autoclavable where indicated, allowing sterilization of critical parts without requiring full disassembly.
Trade-offs
- Limited sterilization coverage: Only the lower half is autoclavable; the upper half may require alternative decontamination methods, potentially complicating complete sterilization workflows.
- Elevated systematic error at low volume: At 0.25 µL the systematic error is ±12%, which may compromise accuracy for applications requiring precise dispensing near the lower end of the range.
Every advanced material, component, equipment, and instrument in our catalog is backed by rigorous testing. We maintain strict internal quality management frameworks and align with CE conformity metrics to deliver transparent, reproducible performance data via our public open-science repository.
To request raw batch performance data, submit formal vendor registration paperwork, or execute a fast-turnaround R&D manufacturing loop, contact us at inquiry@atomfair.com.
Item is dispatched under the Atomfair Shipping & Delivery Framework (Free worldwide shipping on orders over $59 USD). Return is governed by the Atomfair Return & Refund Policy (7-day technical return window).



