Description
Accurate 96-X6 Real-Time Quantitative PCR System, 6 ChannelsProduct Type: Real-Time Quantitative PCR System
Research-grade laboratory product
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LABORATORY PROCUREMENT SUPPORT
For model selection, accessory matching, platform compatibility or configuration confirmation, contact our technical sales team.
E-MAIL: inquiry@atomfair.com
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Supplier: Atomfair
Brand: ATOMFAIR®
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The heated lid can reach temperatures up to 115°C and must not be touched during operation. Reaction volumes must be within 10-50 µL to ensure proper thermal contact and fluorescence signal acquisition.
- Heated Lid Safety: The heated lid can reach temperatures up to 115°C and must not be touched during operation.
- Reaction Volume Constraint: Reaction volumes must be within 10-50 µL to ensure proper thermal contact and fluorescence signal acquisition.
How does the 6-channel fluorescence configuration of the Accurate 96-X6 handle spectral overlap when multiplexing with FAM, Cy5, and ROX in a single run?
The system uses time-resolved real-time scanning with excitation/detection ranges of 455–680 nm and 510–730 nm, respectively, to separate signals across six channels. Each channel is assigned a distinct dye set—F1 for FAM/SYBR Green, F2 for Cy5, F3 for VIC/HEX/JOE/TET, F4 for FAM/SYBR Green, F5 for ROX/Texas Red, and F6 for NED/TAMRA/Cy3—enabling multiplexing without requiring manual spectral compensation. The dynamic range spans 1 to 10^10 copies across 10 quantitative levels, supporting simultaneous quantification of up to six targets.
Can the Accurate 96-X6 be integrated with existing laboratory automation or LIMS systems for high-throughput qPCR workflows?
Data export is supported in CSV, Excel, and text formats via the included software, which provides absolute quantification, relative quantification, qualitative analysis, endpoint fluorescence analysis, melting curve, high-resolution melting curve, and genotyping modes. The standard 96-well format and 10–50 µL reaction volume are compatible with common automation consumables, but no direct LIMS API or robotic arm interface is specified in the documentation. Integration would require custom scripting or middleware to parse exported files.
What are the infrastructure and handling requirements for maintaining temperature uniformity across the 96-well block during long qPCR runs?
The system employs Peltier-based thermal cycling with six independent temperature-control modules and partitioned temperature control with edge compensation to achieve ≤±0.2 °C temperature accuracy and ≤±0.2 °C uniformity across the block. The electronic automatic heated lid is adjustable from room temperature to 115 °C to prevent condensation. Maximum heating/cooling rate is 6 °C/sec over a 4–100 °C range, and the gradient function supports a 1–40 °C difference across 12 columns. No special facility infrastructure beyond standard lab power is required.
This 96-well real-time qPCR system offers six fluorescence channels with long-life LEDs (>100,000 h), high thermal uniformity (≤±0.2 °C), and fast ramp rates (6 °C/sec), making it suitable for multi-plex quantitative PCR. However, users must verify dye compatibility with fixed channel assignments and manage lid temperature settings to avoid evaporation or condensation issues.
Positive
- Six-channel fluorescence with long-life LEDs: Six independent fluorescence channels using single-color high-efficiency LED excitation sources rated for >100,000 hours enable multi-plex qPCR with minimal maintenance downtime.
- High thermal uniformity and fast ramp rate: Temperature accuracy and uniformity ≤±0.2 °C combined with a maximum heating/cooling rate of 6 °C/sec ensure reproducible thermal cycling and rapid protocol completion.
Trade-offs
- Fixed dye-channel assignment constraints: Each of the six channels is pre-assigned to specific dye sets (e.g., F1: FAM/SYBR Green; F2: Cy5); users must confirm their fluorophore compatibility with these fixed assignments before assay design.
- Heated lid temperature management required: The electronic automatic heated lid is adjustable from room temperature to 115 °C; improper lid temperature settings relative to the reaction volume may cause evaporation or condensation, affecting assay accuracy.
Every advanced material, component, equipment, and instrument in our catalog is backed by rigorous testing. We maintain strict internal quality management frameworks and align with CE conformity metrics to deliver transparent, reproducible performance data via our public open-science repository.
To request raw batch performance data, submit formal vendor registration paperwork, or execute a fast-turnaround R&D manufacturing loop, contact us at inquiry@atomfair.com.
Item is dispatched under the Atomfair Shipping & Delivery Framework (Free worldwide shipping on orders over $59 USD). Return is governed by the Atomfair Return & Refund Policy (7-day technical return window).
