Description
Accurate 96-X4 Real-Time Fluorescence Quantitative PCR SystemProduct Type: Temperature control instrument
Research-grade temperature control platform
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TAILORED SOLUTIONS FOR RESEARCH
For bulk orders, OEM requirements, accessory matching or configuration confirmation, contact our engineering sales team.
EMAIL: inquiry@atomfair.com
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Supplier: Atomfair
Brand: ATOMFAIR®
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The instrument requires a stable power supply and an environment free from excessive vibration or direct sunlight to maintain thermal accuracy. The electronic hot lid operates from room temperature to 115 °C and must be properly engaged to prevent condensation and evaporation during cycling.
- Temperature Range Constraint: The system's temperature range of 4 to 100 °C limits its use to protocols that do not require sub-4 °C or above-100 °C incubation steps.
- Sample Volume Constraint: Reaction volumes must be between 10 and 50 µL to ensure proper thermal contact and optical detection within the 96-well block.
- Dye Compatibility Constraint: Only dyes matching the four excitation channels (F1: FAM/SYBR Green, F2: Cy5, F3: VIC/HEX/JOE/TET, F4: FAM/SYBR Green or F5: ROX/Texas Red) are supported for fluorescence detection.
- Block and Module Compatibility Constraint: Only compatible heating blocks, modules, or sample vessels approved by the manufacturer may be used to avoid thermal transfer inefficiency or mechanical damage.
This procedure covers the safe initialization and basic operation of the dry-block thermal cycler for quantitative PCR workflows. Proper setup ensures accurate temperature control and reliable fluorescence data acquisition.
Required Equipment: Accurate 96-X4 Real-Time Fluorescence Quantitative PCR System, Compatible 96-well PCR plate or tube strip, Pipette with sterile filter tips
- Power On and System Check
Connect the instrument to a stable power source and press the power switch to initiate the startup sequence. - Load Sample Vessel
Place the prepared 96-well PCR plate or tube strip into the block, ensuring all wells are seated evenly for uniform thermal contact. - Engage Heated Lid
Close the lid and confirm the electronic hot lid engages automatically, applying pressure and heating to prevent evaporation. - Program Thermal Protocol
Select or enter the desired thermal cycling program via the instrument software, specifying temperature setpoints, hold times, and cycle counts. - Start Run and Monitor
Initiate the run and monitor real-time fluorescence data acquisition through the connected computer interface. - Export Data After Completion
After the run finishes, export raw data in CSV, Excel, or TXT format for downstream analysis.
How does the fourth excitation channel configuration affect multiplexing capability for the Accurate 96-X4 qPCR system?
The fourth channel can be configured for either FAM/SYBR Green (F4) or ROX/Texas Red (F5), but not both simultaneously. This means users must choose between detecting FAM/SYBR Green in two channels (F1 and F4) or adding ROX/Texas Red as a fourth dye while losing the second FAM channel. The system supports 4 excitation channels total, so the trade-off limits multiplexing to either 3 dyes with duplicate FAM or 4 distinct dyes including ROX/Texas Red.
What sample vessel formats and block modules are compatible with the Accurate 96-X4 dry-block temperature control system?
The system is a dry-block temperature control platform designed for 96-well sample capacity with reaction volumes of 10 to 50 µL. However, the product description explicitly states that users must confirm compatible heating blocks, modules, or sample vessels before ordering. The specific block formats (e.g., 0.2 mL tubes, 96-well plates) are not listed in the provided specifications, so compatibility must be verified with the manufacturer based on the intended sample vessel type.
What are the hot lid temperature range and its role in preventing evaporation during qPCR runs on the Accurate 96-X4?
The system features an electronic automatic hot lid adjustable from room temperature to 115 °C. This hot lid applies heat to the top of the reaction vessel to prevent condensation and evaporation during thermal cycling, which is critical for maintaining reaction volume and fluorescence signal integrity. The lid temperature can be set independently of the block temperature (4 to 100 °C) to ensure proper sealing without overheating the sample.
The Accurate 96-X4 is a research-grade real-time PCR system offering four-channel LED excitation and high-sensitivity CMOS detection with single-copy gene sensitivity and 10-order dynamic range, but requires careful block/module compatibility and is limited to 10-50 µL reaction volumes.
Positive
- Four-channel LED excitation with long lifetime: Four monochromatic high-efficiency LEDs provide maintenance-free operation exceeding 100,000 hours, enabling stable fluorescence excitation across multiple channels without frequent replacement.
- High sensitivity and wide dynamic range: Single-copy gene detection sensitivity combined with a 10-order dynamic range (1 to 10^10 copies) allows quantification of both low- and high-abundance targets in the same run.
Trade-offs
- Requires compatible heating blocks and sample vessels: The system relies on specific dry-block modules and sample vessels; users must confirm compatibility before ordering to avoid workflow interruptions.
- Reaction volume limited to 10-50 µL per well: The 96-well format restricts reaction volumes to a narrow range, which may not suit protocols requiring larger or smaller volumes without additional optimization.
Every advanced material, component, equipment, and instrument in our catalog is backed by rigorous testing. We maintain strict internal quality management frameworks and align with CE conformity metrics to deliver transparent, reproducible performance data via our public open-science repository.
To request raw batch performance data, submit formal vendor registration paperwork, or execute a fast-turnaround R&D manufacturing loop, contact us at inquiry@atomfair.com.
Item is dispatched under the Atomfair Shipping & Delivery Framework (Free worldwide shipping on orders over $59 USD). Return is governed by the Atomfair Return & Refund Policy (7-day technical return window).
