Description

Sodium 4-(3-(2-methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2H-tetrazol-3-ium-5-yl)benzene-1,3-disulfonate (CAS: 193149-74-5), also known as WST-8, is a highly sensitive tetrazolium salt widely utilized in cell proliferation and cytotoxicity assays. With the molecular formula C₂₀H₁₃N₆NaO₁₁S₂, this water-soluble compound produces a water-soluble formazan dye upon reduction by cellular dehydrogenases, eliminating the need for solubilization steps. Its optimized structure ensures superior sensitivity compared to traditional tetrazolium salts like MTT, making it ideal for high-throughput screening applications. This reagent is compatible with a broad range of cell types and is particularly valued for its stability and low toxicity. Packaged under strict quality control, our WST-8 is guaranteed to deliver consistent and reproducible results for your research needs.

Properties

• CAS Number: 193149-74-5
• Complexity: 1080
• IUPAC Name: sodium;4-[2-(2-methoxy-4-nitro-phenyl)-3-(4-nitrophenyl)tetrazol-2-ium-5-yl]benzene-1,3-disulfonate
• InChI: InChI=1S/C20H14N6O11S2.Na/c1-37-18-10-14(26(29)30)6-9-17(18)24-22-20(21-23(24)12-2-4-13(5-3-12)25(27)28)16-8-7-15(38(31,32)33)11-19(16)39(34,35)36;/h2-11H,1H3,(H-,31,32,33,34,35,36);/q;+1/p-1
• InChI Key: VSIVTUIKYVGDCX-UHFFFAOYSA-M
• Exact Mass: 599.99814188
• Molecular Formula: C20H13N6NaO11S2
• Molecular Weight: 600.5
• SMILES: COC1=C(C=CC(=C1)[N+](=O)[O-])[N+]2=NC(=NN2C3=CC=C(C=C3)[N+](=O)[O-])C4=C(C=C(C=C4)S(=O)(=O)[O-])S(=O)(=O)[O-].[Na+]
• Topological: 267
• Monoisotopic Mass: 599.99814188
• Synonyms: 193149-74-5, WST-8, 2-(2-Methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium Sodium Salt, 270078-53-0, sodium;4-[2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)tetrazol-2-ium-5-yl]benzene-1,3-disulfonate, MFCD09264687, sodium 4-(3-(2-methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2H-tetrazol-3-ium-5-yl)benzene-1,3-disulfonate, WST-8 Sodium, 2-(3-(2-Methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2H-tetrazol-3-ium-5-yl)-5-sulfobenzenesulfonate, sodium salt, 2H-Tetrazolium,5-(2,4-disulfophenyl)-2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-,inner salt, sodium salt, Sodium 4-[2-(2-methoxy-4-nitro-phenyl)-3-(4-nitrophenyl)tetrazol-3-ium-5-yl]benzene-1,3-disulfonate, sodium 5-(2,4-disulfonatophenyl)-2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-3H-1,2??,3,4-tetrazol-2-ylium, cell proliferation assay, WST-8Sodium, Sodium 4-[3-(2-methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2H-tetrazol-3-ium-5-yl]benzene-1,3-disulfonate, 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2h-tetrazolium,monosodium salt, YSWG295, SCHEMBL21799381, DTXSID30432456, BCP04441, EX-A1809, AKOS015902990, AKOS025311594, AKOS027339261, FM74959, 5-(2,4-Disulfophenyl)-3-(2-methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2H-tetrazolium Inner Salt Sodium Salt, AC-37960, AS-75030, sodium,4-[2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)tetrazol-2-ium-5-yl]benzene-1,3-disulfonate, SY016317, SY233450, A919628, 5-(2,4-disulfophenyl)-3-(2-methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2H-tetrazolium, inner salt, monosodium salt, sodium 4-[3-(2-methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-3H-tetraazol-2-ium-5-yl]-1,3-benzenedisulfonate

WST-8 is extensively used in cell viability and proliferation assays, offering a reliable colorimetric method for quantifying metabolically active cells. It is particularly effective in high-throughput screening due to its water-soluble formazan product, which eliminates the need for organic solvents. Researchers employ WST-8 in drug discovery, toxicology studies, and cancer research to assess cellular responses to various compounds. Its compatibility with multiwell plate readers enhances efficiency in large-scale experiments. Additionally, WST-8 is favored for its minimal interference with cellular metabolism, ensuring accurate and reproducible data.

Safety and Hazards

GHS Hazard Statements

• H314 (95.1%): Causes severe skin burns and eye damage [Danger Skin corrosion/irritation]

Precautionary Statements

• P260, P264, P280, P301+P330+P331, P302+P361+P354, P304+P340, P305+P354+P338, P316, P321, P363, P405, and P501

Hazard Classes and Categories

• Skin Corr. 1B (95.1%)

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Disclaimer: Sold exclusively for laboratory research. Prohibited for commercial use, diagnostics, or human/animal applications. Buyers assume all compliance liability.