Description

Pyroglutamine-4-nitroanilide (CAS: 66642-35-1) is a high-purity biochemical reagent widely utilized in proteolytic enzyme research and diagnostic applications. With the molecular formula C₁₁H₁₁N₃O₄, this compound serves as a chromogenic substrate for detecting pyroglutamyl peptidase and other proteolytic enzymes due to its p-nitroaniline (PNA) leaving group, which releases a yellow-colored product upon cleavage. Ideal for kinetic assays, this substrate is supplied as a crystalline solid with guaranteed >98% purity (HPLC), ensuring reproducibility in enzymatic studies. Its stability under controlled conditions and compatibility with spectrophotometric detection make it a preferred choice for researchers in biochemistry, molecular biology, and pharmaceutical development.

Properties

• CAS Number: 66642-35-1
• Complexity: 358
• IUPAC Name: (2S)-N-(4-nitrophenyl)-5-oxo-pyrrolidine-2-carboxamide
• InChI: InChI=1S/C11H11N3O4/c15-10-6-5-9(13-10)11(16)12-7-1-3-8(4-2-7)14(17)18/h1-4,9H,5-6H2,(H,12,16)(H,13,15)/t9-/m0/s1
• InChI Key: HGNBEWLBSCSJGV-VIFPVBQESA-N
• Exact Mass: 249.07495584
• Molecular Formula: C11H11N3O4
• Molecular Weight: 249.22
• SMILES: C1CC(=O)N[C@@H]1C(=O)NC2=CC=C(C=C2)[N+](=O)[O-]
• Topological: 104
• Monoisotopic Mass: 249.07495584
• Synonyms: 66642-35-1, Pyroglutamine-4-nitroanilide, Pyroglutamyl-p-nitroanilide, 2-Pyrrolidinecarboxamide, N-(4-nitrophenyl)-5-oxo-, (S)-, L-Pyroglutamic acid 4-nitroanilide, (S)-N-(4-Nitrophenyl)-5-oxopyrrolidine-2-carboxamide, 2-Pyrrolidinecarboxamide, N-(4-nitrophenyl)-5-oxo-, (2S)-, (2S)-N-(4-nitrophenyl)-5-oxopyrrolidine-2-carboxamide, PYR-PNA, L-Pyr-pNA, SCHEMBL5839445, Pyroglutamic acid p-nitroanilide, CHEMBL4238582, DTXSID20985224, AKOS027384352, FP48216, AS-70504, CS-0134272, Pyroglutamic acid p-nitroanilide, protease substrate, 2-Pyrrolidinecarboxamide,N-(4-nitrophenyl)-5-oxo-,(2S)-, 5-Hydroxy-N-(4-nitrophenyl)-3,4-dihydro-2H-pyrrole-2-carboxamide

Pyroglutamine-4-nitroanilide is primarily used as a chromogenic substrate to measure pyroglutamyl peptidase activity in vitro, enabling the study of enzyme kinetics and inhibition. It is also employed in high-throughput screening for protease inhibitors and diagnostic assays for enzyme-linked pathologies. The released p-nitroaniline allows for straightforward quantification at 405 nm, facilitating real-time enzymatic analysis.

If you are interested or have any questions, please contact us at support@atomfair.com

Disclaimer: Sold exclusively for laboratory research. Prohibited for commercial use, diagnostics, or human/animal applications. Buyers assume all compliance liability.