Description

Luminol (C₈H₇N₃O₂), also known as 5-amino-2,3-dihydrophthalazine-1,4-dione, is a versatile chemiluminescent compound widely utilized in forensic, biochemical, and analytical applications. This high-purity reagent exhibits exceptional sensitivity in the presence of oxidizing agents, producing a striking blue glow upon reaction, making it indispensable for bloodstain detection and oxidative stress studies. With a molecular weight of 177.16 g/mol and CAS number 521-31-3, our Luminol is rigorously tested for consistency, low impurity levels, and optimal performance in both aqueous and organic systems. Available in crystalline form with >98% purity, it is packaged under inert conditions to ensure long-term stability. Ideal for chemiluminescence assays, forensic investigations, and advanced research in reactive oxygen species (ROS) detection.

Properties

• CAS Number: 521-31-3
• Complexity: 254
• IUPAC Name: 5-amino-2,3-dihydrophthalazine-1,4-dione
• InChI: InChI=1S/C8H7N3O2/c9-5-3-1-2-4-6(5)8(13)11-10-7(4)12/h1-3H,9H2,(H,10,12)(H,11,13)
• InChI Key: HWYHZTIRURJOHG-UHFFFAOYSA-N
• Exact Mass: 177.053826475
• Molecular Formula: C8H7N3O2
• Molecular Weight: 177.16
• SMILES: C1=CC2=C(C(=C1)N)C(=O)NNC2=O
• Topological: 84.2
• Monoisotopic Mass: 177.053826475
• Physical Description: O-aminophthalyl hydrazide appears as yellow crystals or light beige powder.
• Melting Point: 606 to 608 °F
• Solubility: less than 1 mg/mL at 66 °F
• Synonyms: LUMINOL, 521-31-3, 5-Amino-2,3-dihydro-1,4-phthalazinedione, 3-Aminophthalic hydrazide, 3-Aminophthalylhydrazide, NSC 5064, CCRIS 5962, EINECS 208-309-4, UNII-5EXP385Q4F, 5EXP385Q4F, DTXSID5024504, AI3-52555, 1,2-Benzenedicarboxylic acid, 3-amino-, cyclic hydrazide, NSC-5064, DTXCID004504, 3-Aminophthalhydrazide, 5-amino-2,3-dihydrophthalazine-1,4-dione, 1,4-Phthalazinedione, 5-amino-2,3-dihydro-, 5-Amino-1,2,3,4-tetrahydrophthalazine-1,4-dione, 3-Aminophthalic acid hydrazide, MFCD00006890, 5-Amino-2,3-dihydro-phthalazine-1,4-dione, 3-AMINOPHTHALHYDRAZINE, 5-aminophthalazine-1,4-diol, MLS002637790, CHEMBL442329, NSC5064, o-aminophthalylhydrazide, o-Aminophthaloyl hydrazide, 5-Amino-2,3-dihydro-1,4-phthalazinedone, Luminol, 97%, monosodium-alpha-luminol, 3-Aminophthaloylhydrazine, LUMINOL [MI], Luminol, SAJ special grade, Oprea1_698774, Oprea1_819727, SCHEMBL20086, 5-amino-1,4-phthalazinediol, 1, 5-amino-2,3-dihydro-, SCHEMBL2491915, SCHEMBL3051238, Luminol, >=97% (HPLC), 1, 3-amino-, cyclic hydrazide, 3-Aminophthalhydrazide (Luminol), 5-Amino-1,4-dihydroxyphthalazine, HMS3093K04, ALBB-019203, BCP18652, PXB93196, STR04818, Tox21_200940, BBL002758, BDBM50125759, HB0796, s6819, SBB057600, STK138069, STL280212, AKOS000111313, AKOS002945870, AKOS016399881, FL06675, Luminol – for Western Blot and ELISA, SDCCGMLS-0065581.P001, 8-amino-4-hydroxyphthalazin-1(2h)-one, 5-amino-4-hydroxyphthalazin-1(2H)-one, NCGC00091452-01, NCGC00091452-02, NCGC00258494-01, AC-13306, AC-33248, CAS-521-31-3, FL171930, HY-15922, SMR001547307, SY011102, A5301, EU-0066798, NS00032429, ST50447781, EN300-16893, Luminol, for chemiluminescence, >=98.0% (HPLC), Q408061, SR-01000389300, 5-Amino-1,2,3,4-tetrahydro-1,4-phthalazinedione, SR-01000389300-1, BRD-K52559566-001-01-1, BRD-K52559566-001-02-9, Z56813244, F1313-0002, InChI=1/C8H7N3O2/c9-5-3-1-2-4-6(5)8(13)11-10-7(4)12/h1-3H,9H2,(H,10,12)(H,11,13

Application

Luminol is primarily employed in forensic science for latent bloodstain visualization, reacting with hemoglobin’s iron to emit light. In biochemical research, it serves as a sensitive probe for detecting reactive oxygen species (ROS) and oxidative burst in cellular studies. Additionally, it’s used in environmental monitoring to trace metal-catalyzed oxidation processes.

Safety and Hazards

GHS Hazard Statements

• H302 (15.3%): Harmful if swallowed [Warning Acute toxicity, oral]
• H315 (94.9%): Causes skin irritation [Warning Skin corrosion/irritation]
• H319 (94.9%): Causes serious eye irritation [Warning Serious eye damage/eye irritation]
• H335 (93.2%): May cause respiratory irritation [Warning Specific target organ toxicity, single exposure; Respiratory tract irritation]

Precautionary Statements

• P261, P264, P264+P265, P270, P271, P280, P301+P317, P302+P352, P304+P340, P305+P351+P338, P319, P321, P330, P332+P317, P337+P317, P362+P364, P403+P233, P405, and P501

Hazard Classes and Categories

• Acute Tox. 4 (15.3%)
• Skin Irrit. 2 (94.9%)
• Eye Irrit. 2 (94.9%)
• STOT SE 3 (93.2%)

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Disclaimer: Sold exclusively for laboratory research. Prohibited for commercial use, diagnostics, or human/animal applications. Buyers assume all compliance liability.