Description

Isoluminol (CAS 3682-14-2), also known as 6-amino-2,3-dihydrophthalazine-1,4-dione, is a high-purity chemiluminescent compound with the molecular formula C₈H₇N₃O₂. This heterocyclic organic compound is widely utilized in biochemical and analytical applications due to its ability to emit light upon oxidation, making it an essential reagent in chemiluminescence assays. Isoluminol exhibits superior stability and sensitivity compared to its analog luminol, particularly in alkaline conditions. It is commonly employed in immunoassays, DNA detection, and forensic analysis. Our product is rigorously tested for purity (>98%) and is supplied as a fine powder, ensuring optimal performance in research and diagnostic applications. Store in a cool, dry place protected from light to maintain integrity.

Properties

• CAS Number: 3682-14-2
• Complexity: 254
• IUPAC Name: 6-amino-2,3-dihydrophthalazine-1,4-dione
• InChI: InChI=1S/C8H7N3O2/c9-4-1-2-5-6(3-4)8(13)11-10-7(5)12/h1-3H,9H2,(H,10,12)(H,11,13)
• InChI Key: HUDPLKWXRLNSPC-UHFFFAOYSA-N
• Exact Mass: 177.053826475
• Molecular Formula: C8H7N3O2
• Molecular Weight: 177.16
• SMILES: C1=CC2=C(C=C1N)C(=O)NNC2=O
• Topological: 84.2
• Monoisotopic Mass: 177.053826475
• Synonyms: 4-Aminophthalhydrazide, 3682-14-2, Isoluminol, 6-amino-2,3-dihydrophthalazine-1,4-dione, 1,4-Phthalazinedione, 6-amino-2,3-dihydro-, Iso-luminol, 5ID1912ANA, NSC 13567, NSC-13567, UNII-5ID1912ANA, 6-amino-1,2,3,4-tetrahydrophthalazine-1,4-dione, 628-451-4, hudplkwxrlnspc-uhfffaoysa-n, MFCD00010560, 7-Amino-4-hydroxyphthalazin-1(2H)-one, 6-aminophthalazine-1,4-diol, CHEMBL14882, MLS002638327, 6-Amino-2,3-dihydro-1,4-phthalazinedione, 1enu, 7-Amino-4-hydroxy-1(2H)-phthalazinone; 4-Luminol; 6-Amino-2,3-dihydro-1,4-phthalazinedione; Isoluminol; NSC 13567;, 4-Aminophthaloylhydrazine, SCHEMBL28009, 4-Aminophthalhydrazine hydrate, 6-amino-phthalazine-1,4-diol, SCHEMBL2462231, DTXSID70958029, HMS3086N23, NSC13567, BDBM50125767, TD8096, AKOS015916424, AKOS024374898, CS-W017373, DB02041, FI03462, HY-W016657, AC-33250, AS-19110, BP-41760, SMR001547813, SY057321, DB-049010, 6-Amino-2,3-dihydro-phthalazine-1,4-dione, A5300, NS00070453, ST51014901, 6-Amino-2,3-dihydro-1,4-phthalazinedione #, AE-562/42999240, Q27093128

Application

Isoluminol is extensively used as a chemiluminescent substrate in immunoassays (e.g., ELISA) for detecting antibodies or antigens. Its oxidation by horseradish peroxidase (HRP) or other catalysts generates a bright blue light, enabling highly sensitive detection of biomolecules. Researchers also employ isoluminol in forensic science to identify bloodstains via chemiluminescent reactions with hemoglobin. Additionally, it serves as a probe in reactive oxygen species (ROS) studies due to its selective reactivity with peroxides. The compound’s low background signal makes it ideal for high-throughput screening and diagnostic applications.

Safety and Hazards

GHS Hazard Statements

• H315 (100%): Causes skin irritation [Warning Skin corrosion/irritation]
• H319 (100%): Causes serious eye irritation [Warning Serious eye damage/eye irritation]
• H335 (97.5%): May cause respiratory irritation [Warning Specific target organ toxicity, single exposure; Respiratory tract irritation]

Precautionary Statements

• P261, P264, P264+P265, P271, P280, P302+P352, P304+P340, P305+P351+P338, P319, P321, P332+P317, P337+P317, P362+P364, P403+P233, P405, and P501

Hazard Classes and Categories

• Skin Irrit. 2 (100%)
• Eye Irrit. 2 (100%)
• STOT SE 3 (97.5%)

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Disclaimer: Sold exclusively for laboratory research. Prohibited for commercial use, diagnostics, or human/animal applications. Buyers assume all compliance liability.