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Atomfair Fluorescein-5-isothiocyanate FITC C21H11NO5S

Description Fluorescein-5-isothiocyanate (FITC) is a high-purity fluorescent dye widely used in biochemical and cellular research. With the molecular formula C21H11NO5S and CAS number 3326-32-7 , this isomer I derivative of fluorescein isothiocyanate features an isothiocyanate reactive group (-N=C=S) at the 5-position, enabling efficient conjugation to primary amines in proteins, peptides, and other biomolecules. Its excitation/emission maxima (~495/519 nm) make it ideal for fluorescence microscopy, flow cytometry, and immunofluorescence assays. This product is rigorously tested for purity and performance, ensuring optimal labeling efficiency and minimal batch-to-batch variability. Packaged under inert conditions to preserve reactivity, it is suitable for sensitive applications in…

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Description

Description

Fluorescein-5-isothiocyanate (FITC) is a high-purity fluorescent dye widely used in biochemical and cellular research. With the molecular formula C21H11NO5S and CAS number 3326-32-7, this isomer I derivative of fluorescein isothiocyanate features an isothiocyanate reactive group (-N=C=S) at the 5-position, enabling efficient conjugation to primary amines in proteins, peptides, and other biomolecules. Its excitation/emission maxima (~495/519 nm) make it ideal for fluorescence microscopy, flow cytometry, and immunofluorescence assays. This product is rigorously tested for purity and performance, ensuring optimal labeling efficiency and minimal batch-to-batch variability. Packaged under inert conditions to preserve reactivity, it is suitable for sensitive applications in molecular biology, diagnostics, and nanotechnology.

  • CAS No: 3326-32-7
  • Molecular Formula: C21H11NO5S
  • Molecular Weight: 389.4
  • Exact Mass: 389.03579362
  • Monoisotopic Mass: 389.03579362
  • IUPAC Name: 3′,6′-dihydroxy-6-isothiocyanatospiro[2-benzofuran-3,9′-xanthene]-1-one
  • SMILES: C1=CC2=C(C=C1N=C=S)C(=O)OC23C4=C(C=C(C=C4)O)OC5=C3C=CC(=C5)O
  • Synonyms: 3326-32-7, Fluorescein isothiocyanate isomer I, FLUORESCEIN-5-ISOTHIOCYANATE, fluorescein 5-isothiocyanate, Spiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one, 3′,6′-dihydroxy-5-isothiocyanato-

Application

Fluorescein-5-isothiocyanate (FITC) is primarily used to label antibodies, proteins, and nucleic acids for fluorescence-based detection. It enables visualization of cellular structures and biomolecular interactions in techniques like confocal microscopy and ELISA. The dye is also employed in diagnostic assays and drug delivery research due to its bright green emission and stable conjugation chemistry.

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