Description

4-Methylumbelliferyl acetate (CAS No. 2747-05-9) is a high-purity fluorogenic substrate widely used in biochemical and enzymatic research. With the molecular formula C₁₂H₁₀O₄ and IUPAC name (4-methyl-2-oxochromen-7-yl) acetate, this compound is a derivative of 4-methylumbelliferone (4-MU) and serves as a critical tool for detecting esterase and lipase activity. Upon enzymatic hydrolysis, it releases the highly fluorescent 4-methylumbelliferone, enabling sensitive quantification in assays. Ideal for laboratory use, this product is rigorously tested for purity and stability, ensuring reliable performance in diagnostic, pharmaceutical, and research applications. Available in crystalline form, it is soluble in organic solvents such as DMSO and ethanol.

Properties

• CAS Number: 2747-05-9
• Complexity: 345
• IUPAC Name: (4-methyl-2-oxo-chromen-7-yl) acetate
• InChI: InChI=1S/C12H10O4/c1-7-5-12(14)16-11-6-9(15-8(2)13)3-4-10(7)11/h3-6H,1-2H3
• InChI Key: HXVZGASCDAGAPS-UHFFFAOYSA-N
• Exact Mass: 218.05790880
• Molecular Formula: C12H10O4
• Molecular Weight: 218.20
• SMILES: CC1=CC(=O)OC2=C1C=CC(=C2)OC(=O)C
• Topological: 52.6
• Monoisotopic Mass: 218.05790880
• Physical Description: Solid
• Melting Point: 153 – 154 °C
• Synonyms: 7-Acetoxy-4-methylcoumarin, 4-methylumbelliferyl acetate, 2747-05-9, 4-Methyl-2-oxo-2H-chromen-7-yl acetate, 2H-1-Benzopyran-2-one, 7-(acetyloxy)-4-methyl-, beta-Methylumbelliferyl acetate, 7-(Acetyloxy)-4-methyl-2-benzopyrone, 4-methyl-7-acetyloxy coumarin, CHEBI:17763, ZD294D576M, NSC-1059, NSC-31658, NSC-44763, .beta.-Methylumbelliferyl acetate, 7-acetoxy-4-methylchromen-2-one, DTXSID70181895, NSC 1059, EINECS 220-386-6, NSC 31658, NSC 44763, 7-(ACETYLOXY)-4-METHYLCOUMARIN, .BETA.-METHYLUMBELLIFERONE ACETATE, 7-HYDROXY-4-METHYLCOUMARIN ACETATE, 7-Acetoxy-4-methyl-2H-1-benzopyran-2-one, 7-(acetyloxy)-4-methyl-2H-1-benzopyran-2-one, COUMARIN, 7-HYDROXY-4-METHYL-, ACETATE, b-methylumbelliferyl acetate, 4-MUA, b-Methylumbelliferyl acetic acid, 4-Methylumbelliferyl acetic acid, DTXCID10104386, beta-Methylumbelliferyl acetic acid, BETA-METHYLUMBELLIFERONE ACETATE, hxvzgascdagaps-uhfffaoysa-n, (4-methyl-2-oxochromen-7-yl) acetate, Hymecromone Acetate, MFCD00006865, MLS000554751, Acetic acid 4-methyl-2-oxo-2H-chromen-7-yl ester, NSC688806, SMR000146868, ACETYL HYMETOCHROME, cid_366, 7-Acetoxy-4-methyl coumarin, (4-methyl-2-oxidanylidene-chromen-7-yl) ethanoate, CHEMBL12019, SCHEMBL335474, UNII-ZD294D576M, 4-Methyl Umbelliferone Acetate, IFLab1_001442, IFLab2_000119, MEGxp0_001897, 4-Methyl-umbelliferone, acetate, BDBM33456, NSC1059, HMS1416B12, HMS2290M10, ALBB-020396, BCP21289, NSC31658, NSC44763, 4-methyl-2-oxochromen-7-yl acetate, 4-Methylumbelliferyl acetate – 95%, STK395120, AKOS000323400, CCG-214462, NSC-688806, IDI1_019145, (4-methyl-2-oxo-chromen-7-yl) acetate, 4-Methyl-2-oxo-2H-chromen-7-ylacetate, NCGC00246518-01, AC-11030, AS-12521, EM166026, NCI60_000142, NCI60_002720, DB-029341, HY-137877, 4-Methyl-2-oxo-2H-chromen-7-yl acetate #, A1527, CS-0142508, EU-0039270, NS00049036, ST45141012, C03837, 4-Methylumbelliferyl acetate, esterase substrate, AB00448415-08, SR-01000443138, acetic acid (2-keto-4-methyl-chromen-7-yl) ester, SR-01000443138-1, Q27102589, acetic acid (4-methyl-2-oxo-1-benzopyran-7-yl) ester, F0415-0023

Application

4-Methylumbelliferyl acetate is extensively utilized as a fluorogenic substrate for measuring esterase and lipase activity in biochemical assays. Its hydrolysis by these enzymes releases 4-methylumbelliferone, which emits strong fluorescence under UV light, facilitating high-sensitivity detection. This compound is commonly employed in diagnostic kits, drug discovery, and enzyme kinetics studies. Researchers also use it in microbiology to study microbial esterase production. Its stability and reliable performance make it a preferred choice for fluorescence-based enzymatic assays.

Safety and Hazards

GHS Hazard Statements

• H315 (97.6%): Causes skin irritation [Warning Skin corrosion/irritation]
• H319 (97.6%): Causes serious eye irritation [Warning Serious eye damage/eye irritation]
• H335 (95.2%): May cause respiratory irritation [Warning Specific target organ toxicity, single exposure; Respiratory tract irritation]

Precautionary Statements

• P261, P264, P264+P265, P271, P280, P302+P352, P304+P340, P305+P351+P338, P319, P321, P332+P317, P337+P317, P362+P364, P403+P233, P405, and P501

Hazard Classes and Categories

• Skin Irrit. 2 (97.6%)
• Eye Irrit. 2 (97.6%)
• STOT SE 3 (95.2%)

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