Description

4-Chloro-7-nitrobenzofurazan (NBD-Cl) is a highly reactive fluorescent labeling reagent widely used in biochemical and analytical research. With the molecular formula C₆H₂ClN₃O₃ and CAS number 10199-89-0, this compound is a derivative of benzofurazan, featuring a chloro group at the 4-position and a nitro group at the 7-position. Its high reactivity with nucleophiles, such as amines and thiols, makes it an excellent tool for labeling proteins, peptides, and other biomolecules for fluorescence detection. The resulting NBD-labeled compounds exhibit strong fluorescence with excitation/emission maxima around 465/535 nm, making them ideal for fluorescence microscopy, HPLC detection, and other sensitive analytical techniques. This product is available in high purity (≥97% by HPLC) and is suitable for research applications requiring precise and reliable fluorescent tagging.

Key Features:

• High Reactivity: Efficiently labels amines, thiols, and other nucleophiles.
• Strong Fluorescence: Excitation/emission at ~465/535 nm for sensitive detection.
• Versatile Applications: Used in protein labeling, enzyme assays, and cellular imaging.
• High Purity: ≥97% purity by HPLC, ensuring consistent performance.

Properties

• CAS Number: 10199-89-0
• Complexity: 222
• IUPAC Name: 4-chloro-7-nitro-2,1,3-benzoxadiazole
• InChI: InChI=1S/C6H2ClN3O3/c7-3-1-2-4(10(11)12)6-5(3)8-13-9-6/h1-2H
• InChI Key: IGHBXJSNZCFXNK-UHFFFAOYSA-N
• Exact Mass: 198.9784686
• Molecular Formula: C6H2ClN3O3
• Molecular Weight: 199.55
• SMILES: C1=C(C2=NON=C2C(=C1)Cl)[N+](=O)[O-]
• Topological: 84.7
• Monoisotopic Mass: 198.9784686
• Synonyms: 4-Chloro-7-nitrobenzofurazan, Nbd chloride, 4-Chloro-7-nitro-2,1,3-benzoxadiazole, NBD-Cl, 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole, 2,1,3-Benzoxadiazole, 4-chloro-7-nitro-, NBD-chloride, 7-Chloro-4-nitrobenzofurazan, 1-Chloro-4-nitrobenzoxadiazole, NBD-C 1, 7-Chloro-4-nitrobenzo-2-oxa-1,3-diazole, 4-Nitro-7-chlorobenzofurazan, BENZOFURAZAN, 4-CHLORO-7-NITRO-, NBF-Cl, Chloronitrobenzoxadiazole, NSC 228140, CHEBI:78878, UNII-EQF2794IRE, EINECS 233-496-4, EQF2794IRE, 7-Nitro-4-chlorobenzofurazan, BRN 0614212, 4-CHLORO-7-NITROBENZ-2-OXA-1,3-DIAZOLE, NSC-228140, 4-Chloro-7-nitrobenzofurazane, DTXSID2064995, 4-Chloro-7-nitro-benzoxa[1,3]diazole, 7-chloro-4-nitro-2,1,3-benzoxadiazole, 4-chloro-7-nitro-2,1,3-benzooxadiazole, 4-Chloro-7-nitro-benzo[c][1,2,5]oxadiazole, 7-NITROBENZO-2-OXA-1,3-DIAZOL-4-YL CHLORIDE, 4-Chloro-7-nitro-benzoxa(1,3)diazole, 4-Chloro-7-nitro-benzo(c)(1,2,5)oxadiazole, Nitrobenzoxadiazole Chloride, Chloride, NBD, NBF Cl, Chloride, Nitrobenzoxadiazole, 4 Chloro 7 nitrobenzofurazan, 7 Chloro 4 nitrobenzofurazan, DTXCID8032671, ighbxjsnzcfxnk-uhfffaoysa-n, 10199-89-0, MFCD00005808, 4-Chloro-7-nitrobenzo[c][1,2,5]oxadiazole, 4-Chloro-7-nitro-1,2,3-benzoxadiazole, CHEMBL1743207, 4-chloro-7-nitrobenzo[c]1,2,5-oxadiazole, 4-Chloro-7-nitrobenzofurazan; 7-Chloro-4-nitro-2,1,3-benzoxadiazole; NBD-Cl; NSC 228140;, Shokat-update-comp17, nchembio866-comp17, 4-chloro-7-nitrobenzo-2,1,3-oxadiazole, SCHEMBL41754, 4-chloro-7-nitro-benzofurazan, 7-chloro-4-nitro-benzofurazan, SCHEMBL16305393, ALBB-020690, HY-D0042, 4-Chloro-7-nitrobenzofurazan, 98%, BBL011971, BDBM50523948, NSC228140, SBB005345, STK728793, AKOS000121286, AKOS040744095, CS-7530, FC01109, UPCMLD0ENAT5654999:001, 4-chloro-7-nitro 2,1,3-benzoxadiazole, AC-24649, AS-64042, PD063905, ST033521, SY011187, : 4-Chloro-7-nitro-1,2,3-benzoxadiazole, 4-Chloro-7-nitro-2,1,3-benzoxadiazole #, A5592, EU-0033246, NS00023098, EN300-21329, H11879, NBD-Cl;4-Chloro-7-nitro-2,1,3-benzoxadiazole, 7-CHLORO-4-NITROBENZ-2-OXA-1,3-DIAZOLE, Q229939, SR-01000596972, 4-Chloro-7-nitro-1,2,3-benzoxadiazole (NBD-Cl ), SR-01000596972-1, Z104495364, 4-Chloro-7-nitrobenzofurazan, BioReagent, suitable for fluorescence, >=97.0% (HPLC)

4-Chloro-7-nitrobenzofurazan (NBD-Cl) is primarily used as a fluorescent derivatization reagent for amines, thiols, and other nucleophilic groups in biomolecules. It is widely employed in protein and peptide labeling for fluorescence microscopy, flow cytometry, and HPLC-based detection. Researchers also utilize NBD-Cl in enzyme activity assays and as a probe for studying molecular interactions in biological systems. Its stability and bright fluorescence make it a popular choice for sensitive analytical applications.

Safety and Hazards

GHS Hazard Statements

• H301 (23.2%): Toxic if swallowed [Danger Acute toxicity, oral]
• H302 (71.3%): Harmful if swallowed [Warning Acute toxicity, oral]

Precautionary Statements

• P264, P270, P301+P316, P301+P317, P321, P330, P405, and P501

Hazard Classes and Categories

• Acute Tox. 3 (23.2%)
• Acute Tox. 4 (71.3%)

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Disclaimer: Sold exclusively for laboratory research. Prohibited for commercial use, diagnostics, or human/animal applications. Buyers assume all compliance liability.