Description

3,3′-Dimethylbenzidine dihydrochloride (CAS No. 612-82-8) is a high-purity organic compound with the molecular formula C₁₄H₁₈Cl₂N₂. This aromatic diamine derivative is widely utilized in biochemical and chemical research, particularly as a chromogenic substrate in enzymatic assays and immunoassays. Its dihydrochloride salt form enhances solubility in aqueous solutions, making it ideal for laboratory applications requiring precise colorimetric detection. With a purity of ≥99% (HPLC), this reagent is rigorously tested to ensure consistency and reliability in sensitive experiments. Suitable for use in diagnostic kits, histochemistry, and industrial dye synthesis, it is packaged under controlled conditions to maintain stability and performance.

Properties

• CAS Number: 612-82-8
• Complexity: 205
• IUPAC Name: 4-(4-amino-3-methyl-phenyl)-2-methyl-aniline;dihydrochloride
• InChI: InChI=1S/C14H16N2.2ClH/c1-9-7-11(3-5-13(9)15)12-4-6-14(16)10(2)8-12;;/h3-8H,15-16H2,1-2H3;2*1H
• InChI Key: LUKPNZHXJRJBAN-UHFFFAOYSA-N
• Exact Mass: 284.0847040
• Molecular Formula: C14H18Cl2N2
• Molecular Weight: 285.2
• SMILES: CC1=C(C=CC(=C1)C2=CC(=C(C=C2)N)C)N.Cl.Cl
• Topological: 52
• Monoisotopic Mass: 284.0847040
• Physical Description: 3,3′-dimethylbenzidine dihydrochloride is a light tan powder.
• Solubility: 10 to 50 mg/mL at 72 °F
• Synonyms: 612-82-8, o-Tolidine dihydrochloride, 3,3′-DIMETHYLBENZIDINE DIHYDROCHLORIDE, [1,1′-Biphenyl]-4,4′-diamine, 3,3′-dimethyl-, dihydrochloride, DTXSID6020511, DTXCID00511, 5MSK350KD8, UNII-5MSK350KD8, NSC-11223, CCRIS 3495, O-TOLIDINE DIHYDROCHLORIDE [MI], EINECS 210-322-5, NSC 11223, (1,1′-Biphenyl)-4,4′-diamine, 3,3′-dimethyl-, dihydrochloride, 2,3′-Dimethylbiphenyl-4,4′-diamine dihydrochloride, 4,4′-Diamino-3,3′-dimethylbiphenyl dihydrochloride, Dmb Dihydrochloride, DMB2HCl, oTolidine dihydrochloride, 4,4’Biotoluidine dihydrochloride, Benzidine, 3,3’dimethyl, dihydrochloride, 4,4’Diamino3,3’dimethylbiphenyl dihydrochloride, (1,1’Biphenyl)4,4’diamine, 3,3’dimethyl, dihydrochloride, (1,1’Biphenyl)4,4’diamine, 3,3’dimethyl, hydrochloride (1:2), 210-322-5, Benzidine, 3,3′-dimethyl-, dihydrochloride, MFCD00012960, 3,3′-Dimethylbenzidine.2HCl, 4-(4-amino-3-methylphenyl)-2-methylaniline;dihydrochloride, 4,4′-Bi-o-toluidine dihydrochloride, CAS-612-82-8, 3,3′-dimethylbiphenyl-4,4′-diamine dihydrochloride, 4-(4-amino-3-methylphenyl)-2-methylphenylamine, chloride, chloride, (1,1′-Biphenyl)-4,4′-diamine, 3,3′-dimethyl-, hydrochloride (1:2), SCHEMBL867121, CHEMBL1863800, o-Tolidine dihydrochloride, 90%, LUKPNZHXJRJBAN-UHFFFAOYSA-N, EINECS 262-222-6, Tox21_202196, Tox21_302798, SBB003003, AKOS024306953, FS-4077, NCGC00090865-03, NCGC00256319-01, NCGC00259745-01, NS00093474, ST50307644, T0254, D97740, o-Tolidine dihydrochloride, >=99.0% (HPLC), 4,4′-Diamino-3,3′-dimethylbiphenyl Hydrochloride, Q27262572, 2,2′-Dimethyl-1,1′-biphenyl-4,4′-diamine, dihydrochloride

3,3′-Dimethylbenzidine dihydrochloride is primarily used as a chromogenic substrate in peroxidase-based assays, such as ELISA, due to its oxidative conversion to a blue-colored product. It is also employed in forensic chemistry for bloodstain detection and in industrial settings for dye and pigment manufacturing. Researchers value its high sensitivity and reproducibility in quantitative analyses.

Safety and Hazards

GHS Hazard Statements

• H335: May cause respiratory irritation [Warning Specific target organ toxicity, single exposure; Respiratory tract irritation]
• H372: Causes damage to organs through prolonged or repeated exposure [Danger Specific target organ toxicity, repeated exposure]

Precautionary Statements

• P260, P261, P264, P270, P271, P304+P340, P319, P403+P233, P405, and P501

Hazard Classes and Categories

• Acute Tox. 4 (100%)
• Carc. 1B (100%)
• Aquatic Acute 1 (79.2%)
• Aquatic Chronic 2 (100%)

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