Description

1,1′-Di-n-octadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (CAS: 41085-99-8) is a high-purity, lipophilic cyanine dye widely utilized in fluorescence microscopy and cellular labeling applications. With the molecular formula C₅₉H₉₇ClN₂O₄, this compound exhibits strong fluorescence in the red to near-infrared spectrum (ex/em ~549/565 nm), making it ideal for long-term cell tracking, membrane staining, and neuronal tracing. Its amphiphilic structure allows for stable incorporation into lipid bilayers without significant diffusion. The perchlorate counterion enhances solubility in organic solvents, facilitating preparation of stock solutions in DMSO or ethanol. Packaged under inert atmosphere to ensure stability, this product is rigorously tested by HPLC and mass spectrometry to guarantee ≥95% purity. Suitable for use in live-cell imaging, flow cytometry, and histological studies.

Properties

• CAS Number: 41085-99-8
• Complexity: 1210
• IUPAC Name: 2-[3-(3,3-dimethyl-1-octadecyl-indolin-2-ylidene)prop-1-enyl]-3,3-dimethyl-1-octadecyl-indol-1-ium;perchlorate
• InChI: InChI=1S/C59H97N2.ClHO4/c1-7-9-11-13-15-17-19-21-23-25-27-29-31-33-35-41-50-60-54-46-39-37-44-52(54)58(3,4)56(60)48-43-49-57-59(5,6)53-45-38-40-47-55(53)61(57)51-42-36-34-32-30-28-26-24-22-20-18-16-14-12-10-8-2;2-1(3,4)5/h37-40,43-49H,7-36,41-42,50-51H2,1-6H3;(H,2,3,4,5)/q+1;/p-1
• InChI Key: JVXZRNYCRFIEGV-UHFFFAOYSA-M
• Exact Mass: 932.7136873
• Molecular Formula: C59H97ClN2O4
• Molecular Weight: 933.9
• SMILES: CCCCCCCCCCCCCCCCCCN1C2=CC=CC=C2C(C1=CC=CC3=[N+](C4=CC=CC=C4C3(C)C)CCCCCCCCCCCCCCCCCC)(C)C.[O-]Cl(=O)(=O)=O
• Topological: 80.5
• Monoisotopic Mass: 932.7136873
• Synonyms: 41085-99-8, 1,1′-Di-n-octadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate, (2Z)-2-[(E)-3-(3,3-Dimethyl-1-octadecylindol-1-ium-2-yl)prop-2-enylidene]-3,3-dimethyl-1-octadecylindole;perchlorate, 2-[3-(3,3-dimethyl-1-octadecylindol-1-ium-2-yl)prop-2-enylidene]-3,3-dimethyl-1-octadecylindole;perchlorate, DiI perchlorate, DII, DiIC18(3), 1,1′-Dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanin perchlorate, JVXZRNYCRFIEGV-UHFFFAOYSA-M, DTXSID601336591, 1,1 inverted exclamation marka-Dioctadecyl-3,3,3 inverted exclamation marka,3 inverted exclamation marka-tetramethylindocarbocyanine perchlorate, 1,1′-Dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate(DiI), 1,1a(2)-Dioctadecyl-3,3,3a(2),3a(2)-tetramethylindocarbocyanine perchlorate

This dye is extensively used for long-term labeling of cell membranes in fluorescence microscopy due to its exceptional photostability. Researchers employ it for neuronal tracing in developmental neurobiology studies. The compound’s lipophilic nature enables stable incorporation into plasma membranes without intracellular diffusion. It’s also valuable in tracking cell migration and proliferation in vitro and in vivo.

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