Description

Bis(4-nitrophenyl)phosphate (CAS No. 645-15-8) is a high-purity organic phosphate ester with the molecular formula C₁₂H₉N₂O₈P. This compound, also known as bis(4-nitrophenyl) hydrogen phosphate, is a valuable reagent for biochemical and chemical research applications. Its structure features two 4-nitrophenyl groups bonded to a phosphate group, making it useful as a phosphatase substrate, enzyme inhibitor, or synthetic intermediate. Available in crystalline form, this product is rigorously tested for purity and consistency, ensuring reliable performance in sensitive experiments. Ideal for researchers studying enzymatic mechanisms, signal transduction, or phosphate chemistry. Store in a cool, dry place away from light and moisture to maintain stability.

Properties

• CAS Number: 645-15-8
• Complexity: 434
• IUPAC Name: bis(4-nitrophenyl) hydrogen phosphate
• InChI: InChI=1S/C12H9N2O8P/c15-13(16)9-1-5-11(6-2-9)21-23(19,20)22-12-7-3-10(4-8-12)14(17)18/h1-8H,(H,19,20)
• InChI Key: MHSVUSZEHNVFKW-UHFFFAOYSA-N
• Exact Mass: 340.00965225
• Molecular Formula: C12H9N2O8P
• Molecular Weight: 340.18
• SMILES: C1=CC(=CC=C1[N+](=O)[O-])OP(=O)(O)OC2=CC=C(C=C2)[N+](=O)[O-]
• Topological: 147
• Monoisotopic Mass: 340.00965225
• Synonyms: bis(4-nitrophenyl) phosphate, 645-15-8, Bis(4-nitrophenyl) hydrogen phosphate, Bis(4-nitrophenyl)phosphate, bis(p-nitrophenyl) phosphate, Bis(4-nitrophenyl)phosphoric acid, Phosphoric acid, bis(4-nitrophenyl) ester, Phosphoric acid bis(p-nitrophenyl) ester, Bis-4-nitrophenyl phosphate, EINECS 211-434-7, BRN 2064857, bis(p-nitrophenyl) hydrogen phosphate, NSC-59824, Bis[4-nitrophenyl]phosphate, UNII-1MF77LEI63, Phosphoric acid bis(p-nitrophenyl ester), DTXSID20870742, 4-06-00-01330 (Beilstein Handbook Reference), BNPP-4, BpNPP, bis(para-nitrophenol)phosphate, DTXCID40203078, mhsvuszehnvfkw-uhfffaoysa-n, BNPP, bis(p-nitrophenyl)phosphate, di-p-nitrophenyl phosphate, p-Nitrophenyl monohydrogen phosphate, Phosphoric acid, bis(p-nitrophenyl) ester, Phenol, p-nitro-, hydrogen phosphate, NSC 59824, CHEBI:3122, MFCD00007318, Phosphorsaeure-bis-(p-nitro-phenylester), MLS002693253, 1MF77LEI63, CHEMBL1231178, hydrogen di4-nitrophenyl phosphate, Phosphorsaeure-bis-(p-nitro-phenylester) [German], NCIOpen2_007976, Bis(4-nitrophenyl)phosphoric, SCHEMBL223704, HMS3085F04, NSC59824, BDBM50130915, Bis(4-nitrophenyl) phosphate, 99%, SBB067218, AKOS015906713, CS-W013921, DB07418, HY-W013205, Bis(4-nitrophenyl) hydrogen phosphate #, AC-19748, AS-17229, Phosphoric Acid Bis(4-nitrophenyl) Ester, SMR001559212, SY057312, Phosphoric Acid Bis-(4-nitro-phenyl)ester, B1098, NS00079678, ST51006779, C03779, F11268, Q27096640, B4N

Application

Bis(4-nitrophenyl)phosphate is widely used as a chromogenic substrate for alkaline phosphatase assays, where enzymatic hydrolysis releases 4-nitrophenol for spectrophotometric detection. It serves as a model compound for studying phosphatase kinetics and inhibition in biochemical research. The compound is also employed in organic synthesis as a phosphorylating agent or protecting group intermediate. Additionally, it finds use in diagnostic applications and as a reference standard in analytical chemistry.

Safety and Hazards

GHS Hazard Statements

• H300 (95.2%): Fatal if swallowed [Danger Acute toxicity, oral]

Precautionary Statements

• P264, P270, P301+P316, P321, P330, P405, and P501

Hazard Classes and Categories

• Acute Tox. 2 (95.2%)

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