Description

Fluorescein-5-isothiocyanate (FITC) is a high-purity fluorescent dye widely used in biochemical and cellular research. With the molecular formula C₂₁H₁₁NO₅S and CAS number 3326-32-7, this isomer I derivative of fluorescein isothiocyanate features an isothiocyanate reactive group (-N=C=S) at the 5-position, enabling efficient conjugation to primary amines in proteins, peptides, and other biomolecules. Its excitation/emission maxima (~495/519 nm) make it ideal for fluorescence microscopy, flow cytometry, and immunofluorescence assays. This product is rigorously tested for purity and performance, ensuring optimal labeling efficiency and minimal batch-to-batch variability. Packaged under inert conditions to preserve reactivity, it is suitable for sensitive applications in molecular biology, diagnostics, and nanotechnology.

Properties

• CAS Number: 3326-32-7
• Complexity: 668
• IUPAC Name: 3′,6′-dihydroxy-6-isothiocyanato-spiro[isobenzofuran-3,9′-xanthene]-1-one
• InChI: InChI=1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
• InChI Key: MHMNJMPURVTYEJ-UHFFFAOYSA-N
• Exact Mass: 389.03579362
• Molecular Formula: C21H11NO5S
• Molecular Weight: 389.4
• SMILES: C1=CC2=C(C=C1N=C=S)C(=O)OC23C4=C(C=C(C=C4)O)OC5=C3C=CC(=C5)O
• Topological: 120
• Monoisotopic Mass: 389.03579362
• Physical Description: Orange solid; Orange powder;
• Synonyms: 3326-32-7, Fluorescein isothiocyanate isomer I, FLUORESCEIN-5-ISOTHIOCYANATE, fluorescein 5-isothiocyanate, Spiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one, 3′,6′-dihydroxy-5-isothiocyanato-, FITC-I, CHEBI:37918, EINECS 222-042-0, UNII-I223NX31W9, FITC, 5-, DTXSID80892440, Spiro(isobenzofuran-1(3H),9′-(9H)xanthen)-3-one, 3′,6′-dihydroxy-5-isothiocyanato-, 3′,6′-dihydroxy-5-isothiocyanato-3H-spiro[2-benzofuran-1,9′-xanthen]-3-one, 3′,6′-dihydroxy-5-isothiocyanato-3H-spiro[2-benzofuran-1,9′-xanthene]-3-one, FITC-Celite(R), 5 Isothiocyanatofluorescein, UNII-7BUU93N0HM, Fluorescein 5 isothiocyanate, 3′,6′-dihydroxy-4-isothiocyanato-3H-spiro(2-benzofuran-1,9′-xanthen)-3-one, 3′,6′-dihydroxy-4-isothiocyanato-3H-spiro[2-benzofuran-1,9′-xanthen]-3-one, 3′,6′-dihydroxy-5-isothiocyanato-3H-spiro(2-benzofuran-1,9′-xanthen)-3-one, 3′,6′-dihydroxy-5-isothiocyanato-3H-spiro(2-benzofuran-1,9′-xanthene)-3-one, Fluorescein-5(or 6)-isothiocyanate, EINECS 248-207-7, Fluoreszeinisothiocyanat, Fluorescein isothiocyanate isomer I-Celite(R), 7BUU93N0HM, Fluorescein isothiocyanic acid, Fluorescein 5(6)isothiocyanate, DTXCID3029368, CHEBI:37926, Fluorescein5(or 6)isothiocyanate, FLUORESCEIN ISOTHIOCYANATE [WHO-DD], 3′,6’dihydroxy5isothiocyanatospiro(isobenzofuran1(3H),9′(9H)xanthen)3one, 3′,6’Dihydroxy5(or 6)isothiocyanatospiro(isobenzofuran1(3H),9′(9H)xanthene)3one, Benzoic acid, 2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)-, monoisothiocyanato deriv., Spiro(isobenzofuran1(3H),9′(9H)xanthen)3one, 3′,6’dihydroxy5(or 6)isothiocyanato, Spiro(isobenzofuran1(3H),9′(9H)xanthen)3one, 3′,6’dihydroxy5isothiocyanato, 222-042-0, 248-207-7, 25168-13-2, 27072-45-3, fluorescein isothiocyanate, mhmnjmpurvtyej-uhfffaoysa-n, FITC, 5-FITC, 5-isothiocyanatofluorescein, Fluorescein 5(6)-isothiocyanate, 3′,6′-dihydroxy-5-isothiocyanato-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one, FITC isomer 1, MFCD00005063, F5ITC, Fluorescein-5-isothiocyanat, Fluoreszein-5-isothiocyanat, 2-(6-Hydroxy-3-oxo-(3H)-xanthen-9-yl)-5-isothiocyanatobenzoic acid, 3′,6′-dihydroxy-6-isothiocyanatospiro[2-benzofuran-3,9′-xanthene]-1-one, Fluorescein isothiocyanate isomer I, tech grade, I223NX31W9, fluorescein isothiocyanate isomer 1, Fluorescein 5-Isothiocyanate, Isomer 1, 95%, FITC ISOMER I, 3′,6′-dihydroxy-5-isothiocyanatospiro(isobenzofuran-1(3H),9′-(9H)xanthen)-3-one, FITC ISOMER, Fluorescein 5-Isothiocyanate, Isomer 1 (~90%), Fluorescein isothiocyanate isomer I, >=97.5% (HPLC), 5-FITC(isomer I), FITC?, FLUORESCEIN ISOTHIOCYANATE, ISOMER 1, Epitope ID:115575, DSSTox_CID_29368, DSSTox_RID_83483, Fluorescein-4-Isothiocyanate, DSSTox_GSID_49408, SCHEMBL71426, Fluorescein 5-isothiocyanate;, CHEMBL516313, 5(6)-Fluorescein isothiocyanate, FITC, Fluorescein isothiocyanate, Fluorescein-5-isothiocyanate tech., GLXC-10848, BCP04195, EX-A1778, Tox21_202818, 5-FITC;5-Isothiocyanato fluorescein, Fluorescein-5-isothiocyanate (FITC), s6928, AKOS015897625, FITC;5(6)-Fluorescein isothiocyanate, FF05796, FF35415, GS-6079, NCGC00260364-01, AC-37042, AC-37589, BP-23367, BP-30030, DA-53249, Fluorescein isothiocyanate isomer I, 90%, HY-66019, SY011095, CAS-27072-45-3, F0026, NS00028252, ST50826313, A818907, A821696, Fluorescein 5(6)-isothiocyanate, >=90% (HPLC), F04-0012, Q59647575, Fluorescein-5-isothiocyanate; 5-Isothiocyanatofluorescein, 3′,6′-dihydroxy-6-isothiocyanato-1-spiro[isobenzofuran-3,9′-xanthene]one, 3′,6′-dihydroxy-6-isothiocyanato-spiro[isobenzofuran-3,9′-xanthene]-1-one, 12,15-dihydroxy-3-oxospiro[hydroisobenzofuran-1,9′-xanthene]-5-isothiocyanate, 3′,6′-Dihydroxy-5-isothiocyanato-spiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one, 6-isothiocyanato-3′,6′-bis(oxidanyl)spiro[2-benzofuran-3,9′-xanthene]-1-one, Fluorescein isothiocyanate isomer I, suitable for protein labeling, >=90% (HPLC), powder, Fluorescein isothiocyanate isomer I-Celite(R), suitable for fluorescent labeling techniques

Application

Fluorescein-5-isothiocyanate (FITC) is primarily used to label antibodies, proteins, and nucleic acids for fluorescence-based detection. It enables visualization of cellular structures and biomolecular interactions in techniques like confocal microscopy and ELISA. The dye is also employed in diagnostic assays and drug delivery research due to its bright green emission and stable conjugation chemistry.

Safety and Hazards

GHS Hazard Statements

• H334 (100%): May cause allergy or asthma symptoms or breathing difficulties if inhaled [Danger Sensitization, respiratory]

Precautionary Statements

• P233, P260, P271, P284, P304+P340, P342+P316, P403, and P501

Hazard Classes and Categories

• Skin Sens. 1 (100%)
• Resp. Sens. 1 (100%)

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Disclaimer: Sold exclusively for laboratory research. Prohibited for commercial use, diagnostics, or human/animal applications. Buyers assume all compliance liability.