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Atomfair 5-Bromo-6-chloro-3-indolyl-beta-D-galactoside Magenta-GAL C14H15BrClNO6

Description 5-Bromo-6-chloro-3-indolyl-beta-D-galactoside (CAS No. 32137-20-5) is a high-purity chromogenic substrate widely used in molecular biology and biochemical research. This compound, with the molecular formula C14H15BrClNO6, is specifically designed for the detection of beta-galactosidase activity in enzymatic assays, particularly in reporter gene systems like lacZ. The indolyl derivative produces an insoluble magenta-colored precipitate upon cleavage by beta-galactosidase, making it ideal for histochemical staining and colony screening in bacterial and eukaryotic systems. Its high sensitivity and low background interference ensure reliable results in both qualitative and quantitative applications. Store at -20??C protected from light and moisture to maintain stability.

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Description

Description

5-Bromo-6-chloro-3-indolyl-beta-D-galactoside (CAS No. 32137-20-5) is a high-purity chromogenic substrate widely used in molecular biology and biochemical research. This compound, with the molecular formula C14H15BrClNO6, is specifically designed for the detection of beta-galactosidase activity in enzymatic assays, particularly in reporter gene systems like lacZ. The indolyl derivative produces an insoluble magenta-colored precipitate upon cleavage by beta-galactosidase, making it ideal for histochemical staining and colony screening in bacterial and eukaryotic systems. Its high sensitivity and low background interference ensure reliable results in both qualitative and quantitative applications. Store at -20??C protected from light and moisture to maintain stability.

  • CAS No: 32137-20-5
  • Molecular Formula: C14H15BrClNO6
  • Molecular Weight: 408.63
  • Exact Mass: 406.97713
  • Monoisotopic Mass: 406.97713
  • IUPAC Name: (2S,3R,4S,5R,6R)-2-[(5-bromo-6-chloro-1H-indol-3-yl)oxy]-6-(hydroxymethyl)oxane-3,4,5-triol
  • SMILES: C1=C2C(=CC(=C1Br)Cl)NC=C2O[C@H]3[C@@H]([C@H]([C@H]([C@H](O3)CO)O)O)O
  • Synonyms: 93863-88-8, 5-Bromo-6-chloro-3-indolyl-beta-D-galactoside, Magenta-GAL, (2S,3R,4S,5R,6R)-2-[(5-bromo-6-chloro-1H-indol-3-yl)oxy]-6-(hydroxymethyl)oxane-3,4,5-triol, 5-Bromo-6-chloro-3-indolyl b-D-galactopyranoside

Application

5-Bromo-6-chloro-3-indolyl-beta-D-galactoside is primarily used as a chromogenic substrate for beta-galactosidase in lacZ reporter gene assays. It enables visual detection of gene expression in bacterial colonies, yeast, and mammalian cells through the formation of a magenta precipitate. The compound is also employed in histochemistry for localizing beta-galactosidase activity in tissues and embryos. Its high solubility and low spontaneous hydrolysis make it suitable for high-throughput screening applications.

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